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A study was conducted on the diversity and relative abundance of large and medium-sized mammals in the Palo Seco Protective Forest and the Teribe Hydroelectric Power Plant concession area, Bocas del Toro province, Panama, during 2015-2016. Using camera traps and line transects, 45 mammal species were identified, 12 of which are threatened or endangered according to the IUCN and CITES. The monitoring yielded a total of 3,581 photographs, highlighting the Felidae family and key species such as jaguar, puma, and tapir. The study highlights the importance of conservation and the impact of hunting on local communities. Line transects and camera traps were used during a year of monitoring, and 47 species belonging to 18 families and 10 orders were identified. The Felidae group was the most represented among the carnivores. Relative abundance indices (RAIs) were calculated, revealing the Puma concolor as the species with the highest RAI among felines. The study highlights the presence of endangered species such as Panthera onca and Baird's Tapir and emphasizes the need to conserve these habitats.