Secuenciación del gen de la Sialidasa de Trypanosoma rangeli en aislados con y sin actividad enzimática, de la provincia de Panamá Oeste, República de Panamá

Tania Gómez; Gloria González; Franklin Samudio; Carmen Corina de Juncá; Azael Saldaña

Submitted December 13, 2021

Published 2021-12-13

Artículos

Vol. 5 No. 2 (2021): Visión Antataura

Sequencing of Trypanosoma rangeli Sialidase gene in isolates with and without enzymatic activity, of the Province of Panama Western, Republic of Panama

Tania Gómez⁺⁻
Gloria González⁺⁻
Franklin Samudio⁺⁻
Carmen Corina de Juncá⁺⁻
Azael Saldaña⁺⁻

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DOI: ND

Published: 2021-12-13

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Gómez, T., González, G., Samudio, F., Corina de Juncá, C., & Saldaña, A. (2021). Sequencing of Trypanosoma rangeli Sialidase gene in isolates with and without enzymatic activity, of the Province of Panama Western, Republic of Panama. Visión Antataura, 5(2), 20–38. Retrieved from https://revistas.up.ac.pa/index.php/antataura/article/view/2520

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Abstract

Trypanosoma rangeli is a non-pathogenic hemoparasite, common in humans and other mammals. The epimastigotic forms of T. rangeli express an enzyme with sialidase (SA) activity, which is different from the trans-sialidase found in T. cruzi. Two subpopulations of T. rangeli have been described, one with sialidase activity (SA +) and the other without activity (SA-). The objective of this study was to analyze and compare the sequence of the T. rangeli SA gene in SA + and SA- isolates. For this purpose, 2 isolates were analyzed, one SA + from T. rangeli, and one SA-. Sialidase activity was measured using the MU-NANA technique. The total RNA and mRNA of each isolate was extracted, later the SA gene were cloned with pGEM-T Easy and sequenced with 3130 Genetic Analyzer Sanger. It was found that the isolates analyzed have 99% identity with a previously reported sialidase precursor gene, but at the same time it presented differences in 4 amino acid positions.

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