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Knowledge of Mexican nocturnal raptors diet is scarce. We evaluated the diet of the Barn-owl Tyto alba during its breeding season in a small agricultural area the northwestern state of Guerrero, Mexico, from November 2016 to February 2017. We analyzed 24 owl-pellets with 44 prey items from two nests. Small mammals are the principal food item of these birds; represent 48% of the total frequency and 55% of biomass. The trophic feeding overlap index was 83% similitude of the diet among both nests. Further research on pellets at other T. alba nesting sites may help to explain the feeding ecology in an agricultural area, and the potential effects to control crop pests.