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The jaguar is the largest felid in the American continent and is threatened in Panama. Since 1998, we have conducted scientific research to obtain information about the ecology of the jaguar in the Darien National Park and in mixed areas where there are cattle pastures and forest remnants. Based on the information compiled, we learned that 395 jaguars have been killed between 1989 to 2023 in Panama, and Darién is the province where more animals killed were reported. We use camera traps and GPS collars to obtain information about the jaguars, and the information obtained basically is translated for decision making at the level of communities and decision makers. Further, this information is disseminated, good sustainable practices are promoted, and mitigation measures are implemented to promoting the coexistence between the people and jaguars.