The Isla Galeta PPIG Protected Landscape, located in the province of Colón, Panama, houses a high diversity of plants and animals of interest for conservation, However, it is receiving anthropogenic pressures such as the presence of container ports and the expansion of residential projects in the buffer zone. Herpetofauna in this Protected Area has been partially studied and there is no updated inventory of amphibian species. In order to update the lists of amphibians and reptiles in this area, the richness, abundance and conservation status of these groups was determined. For the recording of species, we used the method of searching for transects, close to bodies of water, removing litter, fallen pieces and leaves of the plants. The species accumulation curve was used to assess the degree of confidence in the inventory. It registered 696 individuals and a wealth of 36 species, belonging to 12 species of amphibians and 24 species of reptiles. The study contributes with the knowledge of herpetofaunistic diversity of the province of Colón that can be useful in the Protected Areas Management Plans and the management of forest fragments in urban areas.