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Endophytic fungi are microorganisms that inhabit plants, being a diverse and polyphyletic group, whose ecological relationship with plants is mutualistic and in other cases is negative for the plant. This study presents quantitative data from the evaluation of endophytic fungi in vegetation in the Senderos Río La Villa, El Montuoso Forest Reserve. The El Montuoso Forest Reserve is located in the district of Las Minas, province of Herrera, Republic of Panama. For this reason, the occurrence of mycelial endophytic fungi in vegetation and their relationship in anurans was evaluated by means of leaf sampling and anuran swabs along the trails, and consequent isolation of strains for taxonomic identification. For this, it was sampled in the Senderos Río La Villa. 10 genera were identified: Alternaria sp., Lecythophora sp., Aureobasidium sp., Scedosporium sp., Exophiala sp., Rhinocladiella sp., Curvularia sp., Phialophora sp.The Binomial test to establish a difference in the frequency of occurrence of mycelial endophytic fungi yielded a value of Z = -2.6458, p < 0.05. Of 2 different anuran samples, only 1 presented Helminthosporium sp. and Lecythophora sp. spores. It is concluded that the genus Lecythophora was predominant in the results taken and that the anurans have the capacity to transport spores of endophytic fungi.