This study is an initial evaluation of mollusk biodiversity in the Punta Gorda River basin, during both the 2013 rainy season and the 2014 dry season at eight locations of land, fluvial and coastal habitats. A total of 127 species were collected (62 terrestrials, 18 fluvial and 47 coastal). Field specimens in good part, mostly Mesoamerican endemics, show preference for undisturbed areas. Freshwater species located in swamps or temporarily flooded terrain, are in risk of disappearing due to actual land use. More research must be given to coastal mollusks on account of their ancestral alimentary value for the Rama communities established along the South Caribbean shore.