The orange bellied swamp snake (Tretanorhinus nigroluteus Cope, 1861), is widely distributed from the United States to Colombia (Villa, 1968), in Panama this snake is distributed from the western region to the Costa Arriba de Colon and the ancient Panama Canal Zone (Villa, 1968). Even though in the country specialized inventories have been made to know the species of snakes existing in different protected areas, the Galeta Island Protected Landscape lacks information about reptiles, mainly snakes, except for some informal records. The present note we made the first report of T. nigromaculatus for this protected area, allowing making contributions to the knowledge of reptiles’ fauna that existing in the zone.