Colon University Regional Center (CRUC) has one of the fragments of urban forest of the city of Colon, with high diversity of trees and shrubs, near areas adjacent to the Hydrographic Basin of the Panama Canal (Atlantic Sector); what makes the site an ideal place for studies of diversity and conservation of vertebrates. Between the months of June and August 2016, the first avifauna inventory was carried out in the CRUC, with the objective of estimating the diversity of birds. Using the point-of-counting technique, a total of 1042 individuals of 36 species were registered, distributed in 6 orders and 14 families; Being the most sighted species Quiscalus mexicanus, Thraupis episcopus, Brotogeris jugularis, Turdus grayi, Myiozetetes similis, Sicalis flaveola and Melanerpes rubricapillus. National Law and CITES II list only three of the species in Category VU: Amazona autumnalis, Brotogeris jugularis and Amazilia tzacatl. The species richness and dominance indices (Margalef, Berger Parker) indicate high diversity (5.31) and low dominance (0.14) of birds in CRUC. It is recommended to establish monitoring at other times of the year to estimate changes in the taxonomic composition recorded in this first study.