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A tool was developed that facilitates the determination of the absorbed dose in water under reference conditions (DOSAB) and a dose calculation tool (DOSREL) for the X-RAD 320 preclinical irradiator from Monte Carlo simulations using the DOSXYZnrc code a starting from a point source of isotropic radiation that emits energy spectra generated with the SpekCalc program. This tool can calculate the dose in the central radiation axis and off the axis for different beam qualities and between field sizes of 0.5 x 0.5 and 15 x 15 cm2, for simulated and non-simulated conditions. Relative depth doses and relative dose profiles were also compared for MC simulations and those calculated by linear interpolation for field sizes of 5 x 5 and 7 x 7 cm2 and beam qualities of 300 and 71 kVp and the results shown. a good concordance between both, therefore, the linear interpolation method implemented for intermediate field sizes to those simulated was validated.