The objective of this study was to determine the effect of the supply of calcium salts (PUFA-Ca) of flax oil rich in omega-3 (?-linolenic acid) on the production and chemical composition of milk and its nutraceutical value in dairy cows in early lactation. The experimental period lasted 12 weeks. A total of 36 Holando Argentino cows with 56.4 ± 3.0 days of lactation, 586.8 ± 15.4 kg PV, 2.3 ± 0.2 lactations and 37.7 ± 1.5 kg milk d-1 were used in a randomized complete block design. The treatments were: 1) Omega-3 (O3): 12 kg DM day-1 of alfalfa pasture (Medicago sativa) + 13.5 kg DM day-1 of TMR + 5.2 kg DM of concentrate including 0.85 kg d-1 of PUFA-Ca of flax oil and 2) Control (C): diet similar to O3 but lipid supplementation was replaced by ground corn so that the diets were isoenergetic. No treatment effect was detected (P > 0.05) for any milk production and composition variables, except for urea in milk that was slightly higher in O3 (P = 0.02). The treatment x week interaction was significant (P < 0.05) for production and fat content, with differences only in the 3rd week of the experimental period in favor of group C (1, 39 vs. 1, 13 kg d-1 and 3.86 vs. 3.23% for production and fat content, respectively).