In 2005, a set of practices known as landscape management tools (LMT) was developed in the middle basin of the Barbas river canyon, in the municipalities of Filandia (Quindío) and Pereira (Risaralda), Colombia, to promote the natural habitat, the conservation of native biodiversity and increase the functional connectivity of the landscape. Since its implementation, the effectiveness of biological corridors as habitat for wildlife species has been evaluated, but not yet the contributions that these and the other LMTs could have for the connectivity in the landscape. Through the implementation of computational tools, this research i) analyzed the dynamics of land use between 2003 and 2014, ii) evaluated the configuration and composition of the landscape, and iii) modeled potential movement routes, under two different scenarios, for two species with different levels of mobility and conservation threat: the “cauca guan” (Penelope perspicax) and the “pacarana" (Dinomys branickii). A more connected landscape was found in 2014 compared to 2003, with an increase of 6% of the forest. For the potential connectivity routes, the landscape was shown to be more connected for "cauca guan" than for “pacarana” in all the scenarios. The areas of the landscape that obtained the highest connectivity values coincided with live fences and biological corridors implemented in 2005; however, a single route was identified for both species to displace between the two focal nodes. This route has a critical condition for being surrounded by agricultural activities. This exercise allowed the identification of those sites in the landscape that require the promotion of LMT to reduce pressure from anthropogenic activities, such as paddocks and crops.