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For the first time, the inventory, identification and classification of land molluscs associated with different substrates (trunks, stems, leaves, soil, mosses, leaf litters and branches) is carried out in Darien National Park, Province of Darien. In which 10 species were collected in (41 specimens), grouped into three orders. Nine species were collected in (35 specimens) in the Park and five species (six individuals) outside the Park areas. 10 individuals of Labyrinthus otis orthorhinus represented the highest number of specimens found, followed by Drymaeus mesembrinuseus with seven.