Leiosporoceros dussii (Stephani) Hässel is a neotropical genus of hornwort with little exiting morphological information. This is the only hornwort reported with cyanobacterial colonies present in longitudinally oriented schizogenous canals through the thallus, which is a diagnostic feature for its identification. The aim of this study was to describe the morphology of L. dussii using morphometrical analysis of two populations from El Valle de Anton, in the Cocle Province of Panama. Our analysis showed significant variations in both the gametophyte and the sporophyte when the two populations were compared using multivariate and univariate statistical analysis. This description is a valuable source for the identification of sterile plants of the species Leiosporoceros dussii and expand our knowledge of this phylogenetically important species.