A multitemporal analysis study of changes in vegetation and land use was carried out in five communities located along the Boyd Roosevelt Highway. These communities are part of the vegetation near the watershed of the Panama Canal, which present within their limit’s fragments of forests with a high specific diversity. The vegetation was characterized and quantified taking as input the cartographic information of the periods of 1970, 2000 and 2017 provided by the Ministry of Environment of Panama and photographs of Google Earth. From these data, digital interpretation was performed using the ArcGIS program. The results show that most of the vegetation registered in the study sites corresponds to intervened forests (42%), this suggests a tendency to the progressive loss of the mature forests of the areas. The multitemporal analysis showed that in the 2010-2017 period there was a greater loss of vegetation cover and an increase in urban infrastructures. It also showed that the most deforested areas are mostly in the areas near the provincial capitals; this effect tends to be progressive towards the forests adjacent to the Panama Canal Watershed, which is a threat to the ecological capacity of these forests and therefore to their sustainability faculties.