In this work Artibeus jamaicensis, A. phaeotis, Carollia castanea, C. perspicillata, Chiroderma salvini, Phillostomus hastatus, Sturnira lilium and Uroderma bilobatum are the species of parasited bats by Streblidae, while Artibeus lituratus, A. watsoni and Vampiressa pusilla form the group of the non-parasited bats. Trichobius joblingi is the most abundant Streblidae (53 specimens). We reported for first time in Panama the species Artibeus phaeotis as host for the Neotrichobius stenopterus. Mastoptera guimaraesi, Speiseira ambigua, Strebla mirabilis, Trichobius longipes and T. sparsus are the species of Streblidae reported in the province of Darien.