The mobility systems and public space of hot and humid tropical cities face great challenges, due to the uncomfortable microclimatic conditions, whose building configuration and building materials can generate. In Panama City, the importance of the study of urban qualities, the microclimate, and its influence on the mobility of users in public spaces has been overlooked. This study uses methodologies to measure heat islands in a sector of Panama City and develops an evaluation of design, mobility, and microclimate qualities in the Santa Ana area; site that presented the highest temperatures in the measurement of heat islands. Finally, this study compares and analyzes the mobility, urban design and microclimate qualities that have an impact on the movement of pedestrians and the quality of public space in the Santa Ana sector.