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In the present study, an evaluation has been made comparing the traditional techniques of membrane filtration with the new microbiological analytical procedures of water using the Petrifilm technique, the defined substrate procedure for the fecal and total coliform, and the Colilert test for determining the group of coliforms and fecal coliforms. The samples tested with the three different microbiological evaluations of water, were ob-tained from five rural aqueducts located in the province of Los Santos in October. This research is not experimental and the results obtained from each of the techniques of analyzing water through the Petrifilm technique resulted in a greater number of total coliforms. On the other hand, the defined substrate procedure (Colilert) showed a greater number of fecal coliforms. This was tested through the statistic evaluation, which indicates that there are only significant differences between the Petrifilm technique and the membrane filtration procedure regarding the finding of total coliforms. Nevertheless, the defined substrate procedure is based on the principle of using the defined substrate through which total coliforms and E. coli are simultaneously detected and the variance analyses and correlation as well determine that the former showed more units of colonies of E. coli.