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The presence of microorganisms in endodontic files poses a significant threat to patient safety and the quality of procedures. This study analyzed the presence of microorganisms in new and re-sterilized files used at the Faculty of Dentistry of the University of Panama. A total of 26 files were examined and categorized into six groups based on their usage condition, cleaning process, and sterilization method. The findings revealed that all newly manufactured files contained bacteria, with possible Streptococcus and Staphylococcus identified. Additionally, 50% of re-sterilized files showed contamination, with no significant differences between manual and ultrasonic pre-sterilization cleaning methods. Conversely, unsterilized used files exhibited 100% contamination, highlighting the presence of Streptobacillus. These findings demonstrate deficiencies in both production quality controls and applied sterilization protocols. The presence of microorganisms in re-sterilized files is attributed to the persistence of biological residues acting as protective barriers against autoclave steam. This analysis underscores the importance of establishing mandatory pre-sterilization protocols for new files, as well as combining chemical and physical techniques for cleaning used instruments. Furthermore, continuous training for dental care personnel and the adoption of molecular tools to detect resistant microorganisms are recommended to optimize sterilization standards. These initiatives will help reduce the risk of cross-infections and enhance safety in endodontic procedures at the faculty clinic.
Este obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional.
