This study was carried out in order to determine the diversity of native entomopathogenic fungi associated to insects present in coffee plantations in Boquete (without the presence of Hipothenemus hampei Ferrari). Fieldwork was conducted between October and November 2005. Insects were collected with the symptoms of entomopathogenic fungi attack and were isolated and identified. A total of 33 fungal cultures were obtained from the isolation of mycelia present in samples of insects collected in the field. The cultures correspond to 7 species in 5 genera of pathogenic fungi: Acremonium charticola, A. crassum, Beauveria bassiana, B. brongniartii, Fusarium oxysporum, Lecanicillium tenuipes, Metarhizium anisopliae and Torrubiella tenuis, which were found on insects of the orders Dermaptera and Hemiptera. Of these species, only Beauveria and Acremonium have been documented in other studies as natural enemies of H. hampei. T. tenuis was the only teleomorph found infesting scale insects.