The neotropical genus Varronia P. Browne is one of the most problematic, taxonomically, among the Boraginales. This may be due to the frequency of overlapping the boundaries between intra and interspecific variation of some characters. The purpose of this project was to explore the potential of foliar morpho-anatomic characters for the delimitation of the species found within the Varronia bullata complex in Panama: Varronia inermis (Mill.) Borhidi, Varronia bullata L. and Varronia linnaei (Stearn) J.S. Windmill. For this purpose, mature leaves were collected in different populations for each species. Cross sections were made, and superficial sections of the leaf lamina were obtained, which were used to quantify and qualify previously selected morphoanatomic characters. The quantitative information was subjected to a discriminant canonical analysis. The results of the discriminating canonical analysis that detect the density of simple trichomes in the abaxial epidermis and the length of simple trichomes in the adaxial epidermis, are the characters that have the greatest influence on species separation. On the other hand, a descriptive way is reflected that the presence of crystalline sales, the type of trichomes and the distribution of stomata also contribute to the taxonomic separation of species.