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Fourteen genus and 52 species of Bethylidae were registered from the Darien National Park. The following eigth species of this family were recorded for first time in the Darien National Park: Bakeriella reclusa, Cephalonomia stephanoderis, C. hyalinipennis, Epyris monticola, E. perpolitus, Apenesia crenulata, A. laevigata, Pristocera erythropoda, Prosierola oblicua, Pseudisobrachium petiolatum. General aspects of the biology and diversity of the Bethylidae are mentioned.