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In Panama, the populations of the Geoffroy’s Tamarin (Saguinus geoffroyi) are Near Threatened and as of 2015 a population reduction of 25% is expected. Population ecology studies are key to evaluating the resilience of this primate over time. We aimed to estimate the group structure and population density of S. geoffroyi in the Cerro Ancon Natural Reserve (RNCA). We established a strip transect (1.75 km long and 40 m wide) and traveled it two days a week, in two periods of the day (07:00 a.m. to 12:00 p.m. and 13:00 to 18:00 h) since August 2019 to March 2020. We managed to count 54 individuals divided into nine groups, with an average of 6 ± 0.7 SD (range 5 to 7) individuals/group, with a male:female ratio of 1:0.9, juveniles:female of 1.2:0.9 and infant:female 0.2:0.9. The calculated density was 4.5 individuals/km2. The data are similar to those reported previously, showing resilience of the groups. The RNCA is a refuge area for groups of S. geoffroyi, offering them important food sources. We recommend maintaining forest connections between the surrounding areas and the reserve, thus avoiding overcrowding of groups.