Thirteen genera and forty-nine species of erothylids are reported for Darien National Park, Darien Province, Republic of Panama. The following ten species of erothylids are reported for the first time for Panamá: Erotylus onagga Lacordaire, 1842; Iphiclus conspicillatus Gorham, 1888; I. cordiger Crotch, 1876; Ischyrus palliatus Lacordaire, 1842; Mycotretus interstictus Gorham, 1888; M. lepidus Lacordaire, 1842; M. pecari Lacordaire, 1842; M. scitulus Lacordaire, 1842; M. sericeonitens Crotch, 1876; O. zebra Fabricius, 1787. To comment on the diversity of erotylids of Panama.