This research assesses the potential for improving active mobility on Avenida Cincuentenario in Panama City, within a context of high dependence on private vehicles, congestion, and environmental and public health impacts. The objective is to verify the physical and functional potential of the study area to promote active modes of transportation, particularly cycling, through an analysis of urban morphology, mobility infrastructure, and land use. The methodology combines a literature review with a comprehensive urban diagnosis that considers variables such as built and open spaces, elevation, land use, amenities, block size, road connectivity, topography, accessibility to public transportation, and cycling traffic intensity using data from the Strava platform. The results show a high concentration of trip attractors, connectivity with existing and planned metro stations, significant cycling demand, and a low density of intersections. The study concludes that Avenida Cincuentenario presents favorable conditions for the implementation of active mobility strategies and the design of sustainable cycling infrastructure.