The hematopoietic biostimulation based on minerals (Fe, Cu and Co) and vitamins (B1 and B12) was studied across the first 16 weeks after parturition to evaluate its effects on blood indicators and cellular profiles in lactating Holstein cows in a grade A dairy farm at altitude of 1160 meters. Four lactating Holstein cows at one, two, three and four lactations were used. Two of them by lactation were randomly assigned to a treatment of sterile solution containing [Iron gluconate (10 mg/mL), copper gluconate (10 mg/mL), cobalt gluconate (10 mg/mL), thiamin (100 mg/mL) and cyanocobalamin (20 mg/mL)] and the other two cows by lactation received physiological saline solution (control). Animals were injected for treatment and control 15 ml IM at 10, 40 and 70 days after parturition. The hematopoietic stimulation significantly increased red blood cell count and mean corpuscular hemoglobin concentration (P<.0001) at the 1st and 2nd lactation; showing differences of +7,41% and +8,97%; while, mean corpuscular hemoglobin concentration showed differences +6,17% and +6,21%. However, animals in the 3rd and 4th lactation did not show statistically differences (P>0,05), but were higher. The stimulation increased platelets (P<0,05), White blood cells (P<.01) and neutrophils (P<0,10); however, the accumulated red cell volume, lymphocyte and monocyte were not modified (P>0,05). The hematopoietic stimulation increased some blood cells; indicating that myeloid tissue was activated on its cytogenesis, maturation and liberation of erythrocytes, leukocytes and platelets; as well as hemoglobin across the first third of lactation in Holstein cows under intensive dairy management in the tropics.