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The purpose of our research was to evaluate the pathogenicity of, Heterorhabditis sp. on Agrotis sp. larvae under semi-controlled conditions and field. In the laboratory bioassays, two methods were applied: free (100, 200, 300 NEPs/mL per larva) and capsules (2, 5 and 10 capsules/mL per larva) and a control that consisted of applying 1 mL of distilled water. The field trial was evaluated in plots of 24 m2, for 15 days, where cuttings made to lettuce seedlings by Agrotis sp. were evaluated in different treatments: a control without any application, NEPs, a cultural control (lettuce treated with Chlorpyrifos 75% before planting), and two chemical treatments Engeo (active ingredient Thiamethoxam and Lambda Cyalothrin) and Chlorpyrifos 75%. In this trial, the infestation of Agrotis sp. occurred naturally since it is an endemic pest of the area. The percentage of daily mortality in the laboratory, for both methods, was calculated using the non-parametric survival estimator Kaplan-Meier, which shows that there are significant differences between treatments (P<0.05). Mortality for both application methods was 100% in 5 days of observation. The field trial was analyzed through a generalized linear model adjusted to the Poisson distribution, which shows that there is a difference between treatments (P<0.001), since 28 cuts were observed in the control, 7 and 17 in Chlorpyrifos 75% and Engeo respectively, 3 in the cultural control and 2 with NEPs. These results are interesting because they demonstrate a viable alternative for the biological control of Agrotis sp.
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