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Mapping has advanced significantly thanks to aerial photography, satellite remote sensing, and the use of advanced drones and cameras, improving the collection and representation of geographic data. The tourism industry has benefited from these advances with tourism maps at various scales, which have optimized marketing and transformed tourist habits through mobile devices and digital platforms. However, in the sustainable development plans of tourist destinations, there is still a lack of specific segmentation for alternative tourism, defined by the UN UNWTO as trips focused on natural and cultural activities with a focus on the conservation of resources. The objective of our research was to design a map with segmentations of alternative tourism in order to lay the foundations for the implementation of tourism cartography in the country. In this sense, a geospatial analysis methodology and Geographic Information System (GIS) techniques and tourism field work were implemented. As a result, a map of the Segmentation of alternative tourism in Panama was obtained. The use of technologies such as Geographic Information Systems (GIS), in the cartographic proposal for segmentation of alternative tourism, not only improves tourism planning and management, but also promotes a more sustainable approach; By adopting the methodology used in tourism research, it will be possible to highlight cultural and natural diversity in a responsible and equitable manner in a country or region.