With the aim of raising awareness of the biological, ecological and regional distribution of two species of Battus Scopoli, 1777 (Insecta: Lepidoptera: Papilionidae) in Panama. Various locations in the Republic of Panama (Valle de Anton (Coclé), Darién National Park (Darién), Cerro Alto Higo (Herrera), Jurutungo (Chiriquí) and Soberania National Park (Panama) were sampled. Furthermore, the geographical coordinates of the visited sites were recorded to draw a distribution map of the two species studied. We regularly monitored the sites over a period of two years (2016-2017). In a sampling transect of 1 km, we found the presence of immature and adult stages of the butterflies of the Battus genus, and plants of the Aristolochia (Aristolochiaceae) genus with oviposition by two species of these butterflies. We examined all the plants of the Aristolochiaceae genus found in these transects to detect immature stages of Battus and the presence of any of their parasitoids and predators. The eggs and larvae collected were further transported to the University of Panama to daily monitor them daily in the laboratory. The ecological and biological observations conducted on adult and immature stages of Battus crassus butterfly revealed three cohorts of eggs, larvae, pre-pupae, pupae, and adults of these species. Observations also revealed that B. crassus has four larval stages; their life cycle is approximately of 51 days in laboratory conditions, and one of their natural enemies is a parasitoid of the Diptera order of the Oxysarcodexia sp. (Sarcophagidae) genus. Bio-ecological observations of the Battus polydamas (Linnaeus, 1758) indicate that it takes up to 48 days for to become adults under laboratory conditions. As a result, we predict that the B. polydamas’s full cycle is of about