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Onion (Allium cepa L.) is one of the strategic crops grown in Tierras Altas, Chiriquí, Panama. Although actions have been implemented in terms of research, development and innovation (R&D&I), in response to the needs expressed at the time by farmers, it is necessary to know firsthand the updated agrotechnological demand. The aim of this study was to carry out a participatory rural appraisal (PRA), with actors linked to the onion sector in Tierras Altas. The study site corresponded to Bambito, Tierras Altas, Chiriquí. A total of 80 people participated, including farmers, technicians, researchers, agricultural input sellers, among others. An induction was given to the participants, explaining the reason for carrying out the PRA and a time space was opened for them to express the most urgent needs in terms of R&D&I. According to the results, in consensus the participants mentioned the following as limitations that persist in onion crop in Tierras Altas: i) Pathogens such as Stemphylium pleospora herbarum and Pyrenochaeta terrestris; ii) Thrips tabaci as the main insect pest; iii) Management of water resources in the face of climate change; iv) Agronomic management recommended for commercial cultivars and altitudinal floor; v) Effective weed control using selective herbicides; vi) Improvement of post-harvest management; vii) Recommended cultivars by planting time and photoperiod. In conclusion, the PRA made it possible to identify at least seven relevant aspects to consider in future R&D&I proposals for onion crop in the Highlands.