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The project Design of a Medicinal Trail in the City of the Tree was coordinated by the Historical and Cultural Tourism program at the Regional University Center of San Miguelito between April and September 2022. It arose in response to growing concern over environmental degradation. The primary objective was to develop an interdisciplinary proposal for a medicinal trail that would promote both conservation and educational enjoyment of the natural heritage of the City of the Tree. The project was based on a non-experimental, descriptive, and observational research design. Direct beneficiaries include tourists, local community workers near the City of the Tree, the Office of the Vice-Rector for Extension at the University of Panama, and students from the Central Campus of the San Miguelito Regional Center, who may engage through community service, internships, or thesis work. The findings highlight the City's significant tourism potential, although it currently lacks essential infrastructure, such as accessible trails and structured tourist activities.