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Polychaetes as basic components of the marine benthic food web, ecosystem engineers, and indicators of environmental pollution, in addition to their widespread use in aquaculture projects, encourage the study of the variability of these groups over time. To determine possible changes in the polychaete population on El Agallito Beach, Panama, a comparison of the density, diversity, and structure of the population was carried out between March and July of the years 1997, 1999, and 2021, using samples taken in three stations, with a 17 cm diameter nucleator, introduced 15 cm into the sediment. The rarefaction curve was higher in 1997, and the density fluctuated between 1,423.02 ind/m2 and 5,773.95 ind/m2 during the study. The differences between periods are not related to the granulometry or the amplitude of the tide, however, variations are shown due to the El Niño and La Niña phenomena and their intensity.