Road infrastructures have a great impact on wildlife and landscape: among the impacts we can mention the deaths due to run over of those species of animals that try to cross the road, the creation of a barrier that fragments, disturbs and alters the distribution of the wildlife. Studies of this type are little known in Panama, despite the constant observation of animals killed by collisions on the roads. With the objective of documenting the diversity of mammals that die on the Transístmica highway (Panamá -Colón), bordering fragments of unprotected forest in the country and adjacent to the Panama Canal Watershed; This study was conducted between the months of April and September 2014; Twenty vehicle trips were made both north and south of the road, twice a week during the first hours of the morning and afternoon; A total of 82 run over vertebrates were recorded; 62 of the bodies found could be reliably identified. Of these, 48 correspond to mammals; 6 wild species and 2 domestic species. Of the total of determined species, 1 are in category of threatened by national law, Tamandua mexicana. It is suggested to prepare an annual wildlife death monitoring plan on this road to propose some actions aimed at the conservation of animal diversity.
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