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The present study determined the association between the population density of Dendrobates auratus with environmental and structure factors in five transects in a fragmented tropical forest ecosystem in Bocas del Toro. For this, 25 samplings were carried out between the months of March and July 2023. A total of 138 individuals were recorded with a density of 0.055 individuals/m2. Population density and rainy season were moderately associated with r=0.62. The association of density with the environmental and structural factors of the microhabitats demonstrated significant associations without large variations for both factors: in Water Bodies (Cagua) = 0.861; Bromeliad = 0.808; grassland humidity (HumP) = 0.743; forest humidity (HumB) = 0.709; forest temperature (TB) = 0.681; bamboo= 0.630; trunk= 0.588 and grassland temperature (TP)= -0.547. The canonical correspondence analysis indicated that the population density of D. auratus was dependent on both factors, presenting better associations with nearby water bodies, the number of spatially available bromeliads, forest humidity, and grassland humidity and temperature in inverse direction to the increase in density.