PATOGENICIDAD DE AISLADOS NATIVOS (Beauveria bassiana) Y (Cordyceps javanica) SOBRE LARVAS DE Galleria mellonella (LEPIDOPTERA: PYRALIDAE)

Dustin Moreno-Serrano; Gladys González; Kathia Castrejón; Reynaldo Vargas; Bladimir Xavier Rodríguez-Hernández; Alex Ríos-Moreno

Submitted June 6, 2022

Published 2022-06-06

Artículos

Vol. 4 No. 2: Revista Investigaciones Agropecuarias

PATHOGENICITY OF NATIVE ISOLATES (Beauveria bassiana) AND (Cordyceps javanica) ON LARVAE OF Galleria mellonella (LEPIDOPTERA: PYRALIDAE)

Dustin Moreno-Serrano⁺⁻
Gladys González⁺⁻
Kathia Castrejón⁺⁻
Reynaldo Vargas⁺⁻
Bladimir Xavier Rodríguez-Hernández⁺⁻
Alex Ríos-Moreno⁺⁻

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DOI: ND

Published: 2022-06-06

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Moreno-Serrano, D., González, G., Castrejón, K., Vargas, R., Rodríguez-Hernández, B. X., & Ríos-Moreno, A. (2022). PATHOGENICITY OF NATIVE ISOLATES (Beauveria bassiana) AND (Cordyceps javanica) ON LARVAE OF Galleria mellonella (LEPIDOPTERA: PYRALIDAE). Journal of Agricultural Research, 4(2), 31–43. Retrieved from https://revistas.up.ac.pa/index.php/investigaciones_agropecuarias/article/view/2925

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Abstract

The objective of this work was evaluated of the pathogenic capacity of native isolates of entomopathogenic fungi Beauveria bassiana (IDIAP D-Bb1400) and Cordyceps javanica (IDIAP RS-Cj006), obtained from the Institute of Agricultural Innovation of Panama (IDIAP). The evaluation was carried out through bioassays under laboratory conditions, using V instar larvae of the G. mellonella model insect dipping then in fungal suspensions of 1x10⁷, 1x10⁸and 1x10⁹ conidia ml^-1obtained from a breeding stock of the Faculty of Agricultural Sciences of the University of Panama. For the evaluation, the mortality expressed by the isolates in relation to time was determined, estimating the mean lethal time TL50, and the mean lethal concentration LC50. In all cases, mortality percentages greater than 80% were presented for both isolates and intraspecific differences were observed in the mortality of the treated larvae at concentrations of 1x10⁷, 1x10⁸ y 1x10⁹ conidia ml-1 for both isolates. According to the results obtained, the isolation of Cordyceps javanica was more pathogenic with (TL50: 7.00 days and LC50:1.23x10⁸ conidia ml^-1), although the isolate IDIAP D-Bb1400 also showed excellent potential (TL50: 7.31 days and LC50: 1.55x10⁸ conidia ml^-1). However, there were no significant interspecific differences (p = 0.41) between both isolates and concentrations evaluated (?2 (2) = 0.41, p = 0.81). Through this work, the great pathogenic potential of both isolates was evidenced for the first time in the treated larvae of G. mellonella under laboratory conditions, so it would be important and coherent to carry out field evaluations with these and other native strains, due to their promising potential in pest control in agriculture.

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