We describe richness of non-flying mammals from 14 sites located on the West side of the Panama Canal, specifically in the provinces of Panama, Cocle and Colon. The mammal surveys were done between May 2001 and March 2002. Most of the study site is strongly impacted by human activities. This becomes evident with the high levels of fragmentation in the study area, especially in the low lands. In the high lands the habitats are less disrupted by fragmentation. Small species were collected with Sherman, Tomahauk and Havahart traps. Medium and big size mammals were registered with diurnal and nocturnal surveys, accounting both for the presence and tracks left by the animals. Fourty five non-flying mammalian species were identified, including 6 marsupial species, 5 xenarthrans, 4 primates, 13 rodents, 1 rabbit, 12 carnivores, one tapir and 3 ungulates. Among those species, 28 are categorized under a threatened status. The tapir (Tapirus bairdii), the jaguar (Panthera onca), the puma (Puma concolor), the ocelot (Leopardus pardalis), and the margay (Leopardus wiedii) are among the most threatened species.