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Agricultural activities have the challenge of feeding the growing population without compromising the resources of future generations. Worldwide, livestock represents 40% of total production, so strategies are sought to increase its yield, in this sense the use of improved pastures in cattle feed represents an alternative for weight gain. The objective of this research was to evaluate the use of a formulation of non-symbiotic nitrogen-fixing microorganisms for the production of Cynodon dactylon grass, (L.Pers 1805, Poales,Poaceae). The product used was Bacter Crecimiento® developed by Agrobiologics of Panama (ABP, S.A.). The study site was in the town of Sardina, Corregimiento and District of Penonomé, province of Coclé. Three plots of 40 m2 were measured within an area of one hectare already planted with Cynodon dactylon grass. 3 treatments were applied: Fertilization with Bacter Crecimiento, conventional fertilizer and Absolute Control. On the first day, a leveling cut was made and once the pasture growth cycle was completed, the forage yield was estimated in terms of kg DM/ha. The results obtained indicate a similarity in yield between the plot fertilized with the biological product and the plot with chemical fertilization.