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The quality of the health service perceived by the user is currently gaining more importance since it offers a real picture of the opportunities for improvements in health care. This research aims to: Analyze the user's perception of the quality of service in public health institutions in Panama City.Materials and method: The methodology used was based on a mixed research approach, since it gathers quantitative data which obtains numerical and qualitative data which obtains data from the user's perspective; It is cross-descriptive since it measures the data at a single moment in time, where the variables will be quality of service as an independent variable and user perception as a dependent variable; the research design is non-experimental, since the variables are not manipulated; the sources of information are secondary since it was obtained from information in the database; A study was carried out with the SERVQUAL survey method, which allows measuring user satisfaction, a sample of 75 respondents was taken, techniques were applied; that allowed us to identify the user's perception of the quality of service in public health institutions in Panama City. Results: The result shows that 53.3% of the respondent’s express dissatisfaction according to the quality they perceive with the quality of service in public health institutions in Panama City, where human resources, quality management systems can be some of these causes. Conclusion: With the study of these dimensions through the perception of the user, it was determined that the quality of the service is low and in the same way the variable of user satisfaction.